Email Address:
jsands@emory.edu
Summary of Research Interests:
Dr. Sands' research is directed at understanding the physiology
of the renal inner medulla and the urine concentrating mechanism.
He is currently studying the molecular physiology of urea
transporters and water channels. Dr. Sands uses a combination of
isolated perfused tubule studies to measure transport, antibodies
to measure changes in the amount or location of the transport
proteins, and northern analysis to measure changes in mRNA. Studies
are performed in rats treated to produce physiologic and pathophysiologic
models of human conditions. Dr. Sands
collaborates with several other investigators in the Departments
of Physiology and Pathology at Emory and is a member of a Program
Project Grant entitled: ?Cellular and Molecular Biology of
Renal Transport Processes?. Research areas being addressed include:
1) long-term regulation of urea transport proteins in rat models
of human diseases; 2) mechanisms by which vasopressin
rapidly regulates urea transport; and 3) regulation of urea transporter
promoter activity.
Selected Publications:
Kato A, Sands JM. Evidence for sodium-dependent active urea secretion
in the deepest subsegment of the rat inner medullary collecting
duct. J. Clin. Invest. 101:423-428, 1998.
Kato A, Naruse M, Knepper MA, Sands JM. Long-term regulation of
inner medullary collecting duct urea transport in rat. J. Am. Soc.
Nephrol. 9: in press, 1998.
Sands JM, Flores FX, Kato A, Baum MA, Brown EM, Ward DT, Hebert
SC, Harris HW. Vasopressin-elicited water and urea permeabilities
are altered in the innermedullary collecting duct in hypercalcemic
rats. Am. J. Physiol. 274: in press, 1998.
Terris J, Ecelbarger CA, Sands JM, Knepper MA. Long-term regulation
of renal urea transporter protein expression in rat. J. Am. Soc.
Nephrol. 9: in press, 1998.
Klein JD, Price SR, Bailey JL, Jacobs JD, Sands JM. Glucocorticoids
mediate a decrease in the AVP-regulated urea transporter in diabetic
rat inner medulla. Am. J. Physiol. 273: F949-F953, 1997.
Naruse M, Klein JD, Ashkar ZM, Jacobs JD, Sands JM. Glucocorticoids
down-regulate the vasopressin-regulated urea transporter in rat
terminal IMCDs. J. Am. Soc. Nephrol. 8: 517-523, 1997.
Parker KP, Mitch WE, Stivelman JC, Macon EJ, Bailey JL, Sands JM.
Safety and efficacy of low-dose subcutaneous erythropoietin in hemodialysis
patients. J. Am. Soc. Nephrol. 8: 288-293, 1997.
Rodriguez DF, Kokko JP, Sands JM. Bartter's syndrome, supraventricular
tachycardia, mitral valve prolapse, and asthma: a therapeutic challenge.
Am. J. Med. Sci. 313: 114-116, 1997.
Sands JM, Timmer RT, Gunn RB. Urea transporters in kidney and erythrocytes.
Am. J. Physiol. 273: F321-F339, 1997.
Sands JM, Naruse M, Baum MA, Jo I, Hebert SC, Brown EM, Harris HW.
An apical extracellular calcium/polyvalent cation-sensing receptor
regulates vasopressin-elicited water permeability in rat kidney
inner medullary collecting duct. J. Clin. Invest. 99: 1399-1405,
1997.
Swenson KL, Sands JM, Jacobs JD, Sladek CD. Effect of aging on the
vasopressin and aquaporin responses to dehydration in Fischer 344/Brown-Norway
F1 rats. Am. J. Physiol. 273: R35-R40, 1997.
Sands JM, Kokko JP. Current concepts of the countercurrent system.
Kidney Int. 50: S93-S99, 1996.
Sands JM, Martial S, Isozaki T. Active urea transport in the rat
inner medullary collecting duct: functional characterization and
initial expression cloning. Kidney Int. 49: 1611-1614, 1996.
Sands JM, Naruse M, Jacobs JD, Wilcox JN, Klein JD. Changes in aquaporin-2
protein contribute to the urine concentrating defect in rats fed
a low-protein diet. J. Clin. Invest. 97: 2807-2814, 1996.
Isozaki T. Gillin AG. Swanson CE. Sands JM. Protein restriction
sequentially induces new urea transport processes in rat initial
IMCD. American Journal of Physiology. 266(5 Pt 2):F756-61, 1994
May.
Isozaki T. Lea JP. Tumlin JA. Sands JM. Sodium-dependent net urea
transport in rat initial inner medullary collecting ducts. Journal
of Clinical Investigation. 94(4):1513-7, 1994 Oct.
Tumlin JA. Hoban CA. Medford RM. Sands JM. Expression of Na-K-ATPase
alpha- and beta-subunit mRNA and protein isoforms in the rat nephron.
American Journal of Physiology. 266(2 Pt 2):F240-5, 1994 Feb.
Catudioc-Vallero J, Sands JM, Sidorowicz HF, Klein JD, Sladek CD.
Effect of age and testosterone on the vasopressin response to dehydration
in F344BNF1 male rats. Adv. Exp. Med. Biol. 449: 183-185, 1998.
Kato A, Sands JM. Evidence for sodium-dependent active urea secretion
in the deepest subsegment of the rat inner med-ullary collecting
duct. J. Clin. Invest. 101: 423-428, 1998.
Kato A, Sands JM. Active sodium-urea counter-transport is inducible
in the baso-lateral membrane of rat renal initial inner medullary
collecting ducts. J. Clin. Invest. 102: 1008-1015, 1998.
Kato A, Naruse M, Knepper MA, Sands JM. Long-term regulation of
inner medullary collecting duct urea transport in rat. J. Am. Soc.
Nephrol. 9: 737-745, 1998.
Lea JP, Ertoy D, Hollis JL, Marrero MB, Sands JM. Immunolocalization
of phospholipase C isoforms in rat kidney. Kidney Int. 54: 1484-1490,
1998.
Sands JM, Flores FX, Kato A, Baum MA, Brown EM, Ward DT, Hebert
SC, Harris HW. Vasopressin-elicited water and urea permeabilities
are altered in the inner medullary collecting duct in hypercalcemic
rats. Am. J. Physiol. 274: F978-F985, 1998.
Terris J, Ecelbarger CA, Sands JM, Knepper MA. Long-term regulation
of renal urea transporter protein expression in rat. J. Am. Soc.
Nephrol. 9: 729-736, 1998.
Doran JJ, Sands JM, Timmer RT. Accurate mRNA size determination
in northern analysis using individual lane size markers. BioTechniques
27: 280-282, 1999.
Karakashian A, Timmer RT, Klein JD, Gunn RB, Sands JM, Bagnasco
SM. Cloning and characterization of two new isoforms of the rat
renal urea transporter: UT-A3 and UT-A4. J. Am. Soc. Nephrol. 10:
230-237, 1999.
Kato A, Sands JM. Urea transport pro-cesses are induced in rat IMCD
subseg-ments when urine concentrating ability is reduced. Am.J.Physiol.
276: F62-F71, 1999.
Klein JD, Timmer RT, Rouillard P, Bailey JL, Sands JM. UT-A urea
transporter expressed in liver: up-regulation by uremia. J. Am.
Soc. Nephrol. 10: 2076-2083, 1999.
Sands JM. Urea transport: it's not just "freely diffusible"
anymore. News Physiol. Sci. 14: 46-47, 1999.
Sands JM. Regulation of renal urea transporters. J. Am. Soc. Nephrol.
10: 635-646, 1999.
Timmer RT, Sands JM. Lithium intoxication. J.Am.Soc.Nephrol. 10:
666-674, 1999.
Sands JM. The medullary collecting duct urea transporter. Curr.
Opin. Nephrol. Hypertens. 8: 499-504, 1999.
Catudioc-Vallero J, Sands JM, Klein JD, Sidorowicz HE, Sladek CD.
Effect of age and testosterone on the vaso-pressin and aquaporin
responses to dehydration in Fischer 344/Brown-Norway F1 rats. J.
Gerontology: Biol. Sci. 55A: B26-B34, 2000.
Kato A, Klein JD, Zhang C, Sands JM. Angiotensin II increases vasopressin-stimulated
facilitated urea permeability in rat terminal IMCDs. Am. J. Physiol.
279: F835-F840, 2000.
Nakayama Y, Peng T, Sands JM, Bagnasco SM. The TonE/TonEBP pathway
mediates tonicity-responsive regulation of UT-A urea transporter
expression. J. Biol. Chem. 275: 38275-38280, 2000.
Sands JM. Regulation of urea transporter proteins in kidney and
liver. Mt. Sinai J. Med. 67: 112-119, 2000.
Sands JM, Layton HE. Urine concentrating mechanism and its regulation;
in Seldin DW, Giebisch G (eds): The Kidney: Physiology and Pathophysiology,
3rd edition. Philadelphia, Lippin-cott Williams and Wilkins, 2000,
pp. 1175-1216.
Nakayama Y, Naruse M, Karakashian A, Peng T, Sands JM, Bagnasco
SM. Cloning of the rat Slc14a1 gene and genomic organization of
the UT-A urea transporter. Biochim. Biophys. Acta 1517: in press,
2001.
Sands JM. Milestones in Nephrology Guest Commentary on: Bodil Schmidt-Nielsen,
"Urea excretion in white rats and kangaroo rats as influenced
by excitement and by diet". J. Am. Soc. Nephrol. 12: in press,
2001.
Bagnasco SM, Peng T, Janech MG, Karakashian A, Sands JM. Cloning
and characterization of the human urea transporter UT-A1 and mapping
of the human Slc14a2 gene. Am.J.Physiol. - Renal Physiol. 281: in
press, 2001.
Bradford AD, Terris JM, Ecelbarger CA, Klein JD, Sands JM, Chou
C-L, Knepper MA. 97 and 117 kDa forms of the collecting duct urea
transporter UT-A1 are due to different states of glycosylation.
Am.J.Physiol. - Renal Physiol. 281: in press, 2001.
Duchesne R, Klein JD, Velotta JB, Doran JJ, Rouillard P, Roberts
BR, McDonough AA, Sands JM. UT-A urea transporter protein in heart:
increased abundance during uremia, hypertension, and heart failure.
Circ. Res. 89: in press, 2001.
Nakayama Y, Naruse M, Karakashian A, Peng T, Sands JM, Bagnasco
SM. Cloning of the rat Slc14a2 gene and genomic organization of
the UT-A urea transporter. Biochim. Biophys. Acta Gene Struct. Expression
1518: 19-26, 2001.
Sands JM. Urinary concentration and dilution; in Massry SG, Glassock
RJ (eds): Textbook of Nephrology, 4th edition. Baltimore, Lippincott
Williams and Wilkins, 2001, pp. 104-109
Sands JM. Milestones in Nephrology Guest Commentary on: Bodil Schmidt-Nielsen,
"Urea excretion in white rats and kangaroo rats as influenced
by excitement and by diet". J.Am.Soc.Nephrol. 12: 856-864,
2001.
Sands JM. Urine concentrating mechanism: measured permeability values
in medullary nephron segments and urea transport processes. Proceedings
of the IMA workshop on Membrane Transport and Renal Physiology,
Minneapolis, MN, 2001, in press.
Timmer RT, Klein JD, Bagnasco SM, Doran JJ, Verlander JW, Gunn RB,
Sands JM. Localization of the urea transporter UT-B protein in human
and rat erythrocytes and tissues. Am.J.Physiol. - Cell Physiol.
281: in press, 2001.
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